Three Sisters Springs Wildlife Refuge

Enjoy a boardwalk around Three Sisters Springs and explore 57 acres with nature trails to view 5 natural springs, Lake Crystal and wetlands. Manatee can be found in Kings Bay year round.

Please note that there is no public parking or water access on the refuge. During the summer season (April 1st-Nov. 14th ), walk-in/bicycle-in access is available seven days a week from the front gate located at 601 Three Sisters Springs Trail, Crystal River,Fl. Front Gate accepts only credit and debit card for payment.

Trolley Tours will operate on Friday, Saturday and Sunday from the Three Sisters Springs Center located at 123 NW US Hwy 19, Crystal River, Florida (Behind City Hall). At the Center, you will purchase your tickets and ride the trolley to Three Sisters Springs.